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Effect of the length of the homologous arms on editing efficiency. (A) Schematic of the donor templates with different lengths of homologous arms flanking the amber mutation site. (B) Plating of the phage lysates from the donor DNA templates shown in panel A. (C) The number of recombinant plaques produced using donor templates of different lengths. “∗” and “∗∗” indicate p < 0.05 and <0.01, respectively (ANOVA).
