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. Author manuscript; available in PMC: 2018 Jan 17.
Published in final edited form as: ACS Synth Biol. 2017 Jul 13;6(10):1952–1961. doi: 10.1021/acssynbio.7b00179

Figure 7.

Figure 7

The phage T4 rnlB is a nonessential gene. (A) Schematic showing the introduction of a 437-bp deletion into rnlB region using CRISPR-Cas9 genome editing. Protospacer is shown in black and Hoc gene is shown in orange. Two stop codons were also introduced following the deletion. The line shows the deleted region. (B) Spot-testing of an rnlB deletion mutant plaque shows that it grows on both E. coli B834 (sup0) and CR63 (sup1). WT phage was used as a control (blue arrow). (C) PCR demonstrates that the rnlB mutant plaque has a deletion of appropriate size. (D) One-step growth curves of rnlB deleted T4(C) and T4(C) phages on E. coli DH5α.