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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Neuropharmacology. 2017 Aug 10;125:365–375. doi: 10.1016/j.neuropharm.2017.08.008

Table 2.

Allosteric operational model parameters for PSNCBAM-1, RTICBM-15, and RTICBM-28 in CP55,940-stimulated [35S]GTPγS binding.

Compound Calculated affinity and cooperativity factors
alpha ± SEM 95% C.I. beta ± SEM 95% C.I.
PSNCBAM-1 55.01 ± 3.76 (47.65 – 62.37) 0.003 ± 0.001 (0.0003 – 0.005)
RTICBM-15 20.71 ± 1.43 (17.91 – 23.51) ~0 ND
RTICBM-28 201.1 ± 11.95 (177.7 – 224.5) 0.001 ± 0.001 (0 – 0.003)

Data were fit to allosteric operational model (see equation 2) with parameters constrained as follows: logKA=−6.6; Emax=100; TA=14; TB=0; n=1; α>0; β>0. LogKB was constrained individually to values obtained from [3H]SR141716 binding data (Table 4). Values considered significant by non-overlapping 95% confidence intervals. ND = not determined.