Fig. 2.

The effect of EGCG and pioglitazone on mRNA level of G6Pase. HepG2 cells were incubated for 24 h with palmitate (0.5 mM), 10 μM pioglitazone and 40 μM EGCG. Total RNA extracted, and then, the level of G6Pase expression was determined by real-time PCR and normalized to endogenous β-actin. *p < 0.05 versus untreated cells, **p < 0.05 versus just palmitate-treated cells, ≠<0.05 Combined treated cells versus the cells individually treated with palmitate and EGCG or Pioglitazone. The figure shows representative data gained from mean ± SD of three independent experiments.