Fig. 3.

RNA-seq analysis of retinal gene expression. RNA-seq experiments were performed on retinal samples collected from Abca4^−/−Rdh8^−/− mice. Two groups were compared: one unexposed to bright light (control) and light-exposed mice pretreated with DMSO (Light) or with either MTP, TAM, and BRM or MTP, DOX, and BRM (n = 3/group). (A) Normalized fragments per kilobase of genes after single drug treatments. Total RNA was subjected to RNA-seq analysis, and FPKM, normalized FPKM, were calculated. (B) Normalized fragments per kilobase of genes after combined drug treatments. Combined drug treatments consisted of either MTP + TAM + BRM or MTP + DOX + BRM. Drug doses for monotherapy were as follows: 10 mg/kg MTP, 2.5 mg/kg TAM, 10 mg/kg DOX, and 1 mg/kg BRM. Doses for combination therapy were as follows: 1 mg/kg MTP, 0.05 mg/kg TAM, 1 mg/kg DOX, and 0.1 mg/kg BRM. FPKM, fragments per kilobase of transcript per million mapped reads.