Fig 6.

Injection of the PC61 antibody decreases the tumor number by the increased percentage of CD4⁺ and CD8⁺ cells during CAC. Mice that were treated as described in figure 5 were sacrificed 73 days after administration of AOM. A) Representative data of the colon morphology of the different groups. Total data of the B) length of the colon and C) number of tumors in the different groups. D) H&E histology to detect morphological changes in the different groups. E) Confocal immunofluorescence of the different groups showing the expression of green fluorescent protein in the cells (Tregs) from the colon tissue. F) Representative dot plot data and G) total percentage of CD4⁺ and CD8⁺ T cells and CD8⁺CD25⁺ T cells from the MLN of the different experimental groups. The lymphocyte region was first defined by FSC and SSC characteristics and further subgated based on CD3 and CD4 expression. Five thousand events from either subgate were captured. Data from 2 experiments with at least 3 mice per group per day of the analysis. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA and Tukey's multiple comparison test.