A : The expression of ERα、p-Akt and nuclear NF-κB p65 proteins of HeLa cells treated with Gen; B: The effect of Gen on the expression of ERα protein. *p<0.05 vs control group (t=0), n=3. C: The effect of Gen on the expression of p-Akt protein. *p<0.05 vs control group (t=0), n=3. D: The effect of Gen on the expression of nuclear NF-κB p65 protein.*p<0.05 vs control group (t=0), n=3. Gen induced activation of ERα, PI3K/Akt and nuclear NF-κB p65 proteins in HeLa cells. HeLa cells were treated with Gen (0.1 μmol‧L-1) for increasing time points (0, 0.5, 1, 2 and 3 h). Time course study of protein expression of ERα、p-Akt and nuclear NF-κB p65 by Western blotting.