. 2018 Jan 1;9(2):288–295. doi: 10.7150/jca.20499

Table 1.

Primers used for RT-PCR

Primer Sequence		Size of product	Tm (ºC)	Cycles
β-actin	Forward:5'-CATGTACGTTGCTATCCAGGC-3'	250 bp	60	30
β-actin	Reverse:5'-CTCCTTAATGTCACGCACGAT-3'	250 bp	60	30
Bcl-2	5'-CGACGACTTCTCCCGCCGCTACCGC-3'	319 bp	60	30
Bcl-2	5'-CCGCTAGCTGGGGCCGTACAGTTCC-3'	319 bp	60	30
Cyclin-D1	5'-GTGAAGTTCATTTCCAATCCGC-3'	167 bp	60	30
Cyclin-D1	5'-GGGACATCACCCTCACTTAC-3'	167 bp	60	30
VEGF	5'-ATGGCAGAAGGAGGAGGG-3'	420 bp	60	30
VEGF	5'-CGAAACGCTGAGGGAGGCT-3'	420 bp	60	30
u-PA	5'-TCACCACCAAAATGCTGTGT-3'	231 bp	60	30
u-PA	5'-AGGCCATTCTCTTCCTTGGT-3'	231 bp	60	30
TRAF	5'-AGAGGCCCCAGAGTGAAACC-3'	294 bp	60	30
TRAF	5'-AGATGGCCAGCCCGAAGTCG-3'	294 bp	60	30
c-IAP1	5'-GAAGACATCTCTTCATCGAGG-3'	688 bp	60	30
c-IAP1	5'-CCACAGGTGTATTCATCATGAC-3'	688 bp	60	30
c-IAP2	5'-ACAGGAAGCAAAGCACAGG-3'	138 bp	60	30
c-IAP2	5'-CTGTCCTAGACTTGTTCCGTTA-3'	138 bp	60	30