Figure 3.

MiR-378 inhibitor- and miR-1827 mimic-treated A549-I7 cells mitigated tumor angiogenesis in embryonic zebrafish. (A) Representative images of the zebrafish embryos subjected to whole-mount ALP staining. Arrows in black depict the ectopic vessel(s) induced by tumor cells implanted. (B) Quantification of number of ectopic vessels originating from the developing SIV in each embryo of four treatment groups. Scrambled inhibitor- and scrambled mimic-treated cells induced robust neovascular response, whereas miR-378 inhibitor- and miR-1827 mimic-treated cells were less angiogenic. (C) Quantification of length of ectopic vessels originating from the developing SIV in each embryo of four treatment groups. N_{MiR-378 inhibitor-treated} = 44; N_{Scr inhibitor-treated} = 30; N_{MiR-1827 mimic-treated} = 30; N_{Scr mimic-treated} = 34