Figure 2.

The effect of miR-675 on the proliferation and cell cycle distribution of PDAC cells, and the suppression of cell proliferation caused by H19 knockdown could be rescued by inhibiting miR-675. AsPC-1 and PANC-1 cells were transfected with miR-675 mimic, miR-675 inhibitor, siH19, siH19+miR-675 inhibitor or corresponding negative controls respectively. (A) The transfection efficiency for each group was verified by qPCR. (B) Cell viability was determined by the CCK-8 assay. The cell proliferation curves were drawn based on the measured absorbance at 450 nm every 24 h after transfection. (C) The colony-forming ability of the transfected cells was examined by colony formation assay. The numbers of macroscopic colonies were counted after 2-3 weeks. (D) Cells in S phase (which could incorporate the EdU reagent) were detected by fluorescence microscopy and counted. (E) The distribution of cell cycle progression was determined by flow cytometry. The results were expressed as the mean ± s.d., n=3, *P<0.05, **P<0.01.