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. 2018 Jan 1;9(2):389–399. doi: 10.7150/jca.21347

Figure 4.

Figure 4

Regulation of miR-675 on PDAC cell cycle was mediated via direct inhibition of E2F-1 translation. (A) Bioinformatic miRNA target prediction software indicated that there were two putative miR-675 binding sites in the 3'UTR of the E2F-1 mRNA. (B) Luciferase reporter plasmids containing the wild-type or mutated 3'UTR fragments were co-transfected with either miR-675 mimic or negative control. The intensity of luciferase activity was measured using luminescence. (C) The protein levels of E2F-1 in the cells transfected with miR-675 mimic, miR-675 inhibitor, siH19, siH19+miR-675 inhibitor or corresponding negative controls were determined by western blot analysis. The results were expressed as the mean ± s.d., n=5, *P<0.05, **P<0.01.