Antitumor activities of LCL-SIM and free SIM in C26 colon carcinoma-bearing mice. Mice received two i.v. injections of either formulation at day 8 and day 11, after cell inoculation. The data were reported at day 12 after tumor cell inoculation (the day when mice were sacrificed) A. Tumor volumes at day 12 in different treatment groups. B. Areas under the tumor growth curves (AUTC) until day 12. The results were expressed as mean ± SD of five mice; Control - untreated group (group treated with PBS); SIM - group treated with 5 mg/kg free SIM at days 8 and 11 after tumor cell inoculation; LCL-SIM - group treated with 5 mg/kg LCL-SIM at days 8 and 11 after tumor cell inoculation. To compare the effects of each treatment on tumors in vivo with controls (tumors in mice treated with PBS), one-way ANOVA with Dunnett's Test for multiple comparisons was used and the p values are indicated as follows: ns, not significant (p>0.05); *, p<0.05; **, p<0.01.