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. 2018 Jan 1;9(2):440–449. doi: 10.7150/jca.21560

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Immunohistochemical analysis of the cytotoxic effects of different treatments on C26 murine colon carcinoma in vivo. A-C. PCNA expression in tumors. Cells that present nuclear staining in brown are PCNA-positive cells. Sections were counterstained with Gill 2 hematoxylin. D-F. Bax expression in tumors. Cells that present cytoplasmic staining in brown are Bax-positive cells. Sections were counterstained with Gill 2 hematoxylin. A and D-Control (untreated group); B and E- group treated with 5 mg/kg SIM at days 8 and 11 after tumor cell inoculation; C and F- group treated with 5 mg/kg LCL-SIM at days 8 and 11 after tumor cell inoculation. Scale bars of 20 µm and original magnification of 400X.