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. 2017 Nov 11;17(1):e12679. doi: 10.1111/acel.12679

Figure 3.

Figure 3

SIRT3 prevents p53‐induced mitochondrial and DNA damage in primary cortical neurons. (A) Photomicrographs show that SIRT3 prevents mito‐p53‐induced neuronal damage. DIV7 primary mouse cortical neurons were transfected with pDsRed control vector or pDsRed mito‐p53 with SIRT3 or ΔSIRT3 for 36 h. Primary neurons were treated with 0.1% saponin (5 min on ice) to release cytosolic content prior to fixation and were subjected to immunostaining for cytochrome c staining and nuclear counterstaining with DAPI. Images were captured by confocal microscopy. Representative images of four separate experiments are shown. Scale bar (white): 10 μm. (B) SIRT3 prevents mito‐p53‐induced neuronal DNA damage. The DNA fragmentation was analyzed from transfected neurons and the bar graphs represent the mean ± SEM (n = 4; *P < 0.05). (C) SIRT3 prevents mito‐p53‐induced mitochondrial damage. The bar graphs represent relative endogenous cytochrome c (FITC) intensity that is quantified using Nikon NIS‐Elements AR software. Error bars represent S.E.M (n = 4; **P < 0.01, ***P < 0.001).