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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Dec 20;495(3):2363–2368. doi: 10.1016/j.bbrc.2017.12.107

Figure 1. USP36 Deubiquitinates H2Bub1.

Figure 1

(A) USP36 deubiquitinates H2Bub1 in cells. H1299 cells transfected with increasing doses of Flag-USP36 were assayed by IB. (B) USP36-mediated reduction of H2Bub1 requires its catalytic activity. H1299 cells transfected with Flag-tagged wt USP36 or its catalytically inactive C131A mutant were assayed by IB. (C) USP36 deubiquitinates H2Bub1 in vitro. Histones purified from H1299 cells were incubated with wt His-USP361-800 or the His-USP361-800/C131A mutant purified from bacteria and assayed by IB.