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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Dec 20;495(3):2363–2368. doi: 10.1016/j.bbrc.2017.12.107

Figure 2. USP36 interacts with H2B.

Figure 2

(A). USP36 binds to H2B in vitro. Purified histones were incubated with GST-USP361-800 or GST only, followed by IB with anti-H2B antibody. (B) USP36 binds to H2Bub1 in vitro. Purified histones were incubated with GST-USP361-800, GST-USP361-800/C131A or GST only, followed by IB. (C) USP36 interacts with H2B in cells. H1299 cells transfected with V5-USP36 alone or together with Flag-H2B were subjected to co-IP with anti-Flag followed by IB. (D). USP36 associates with chromatin. Purified histones were treated without or with Mnase and assyed by IB. The soluble (S) and insoluble (P) fractions are indicated.