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. Author manuscript; available in PMC: 2018 Jul 3.
Published in final edited form as: Nature. 2018 Jan 3;553(7687):222–227. doi: 10.1038/nature25171

Figure 2. Phosphorylation of PIN4 at Y122 affects mitochondrial metabolism.

Figure 2

a, Immunoblot of phosphotyrosine immunoprecipitates from SF126 glioma cells (left panels). Right panels, whole cell lysates (WCL). Paxillin is loading control. b, Quantification of pY122-PIN4 integrated mean fluorescence intensity (IMFI) from F3-T3-positive and F3-T3-negative GBM. Box plot spans the first quartile to the third quartile and whiskers show the 1.5× interquartile range. P≤0.0001, two-sided Mann-Whitney test. c, OCR of HA-F3-T3 transduced with PIN4 WT, PIN4-Y122F or vector. d, OCR of HA-F3-T3 following silencing of PIN4 and reconstitution with PIN4-WT or PIN4-Y122F. e, Soft agar colony forming assay of HA treated as in d. Data in c-e are each Mean±s.d. of one representative experiment with n=3 technical replicates. Experiments were repeated three times with similar results. **P≤0.01, ***P≤0.001, two-tailed t-test, unequal variance.