Skip to main content
. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Circ Arrhythm Electrophysiol. 2018 Jan;11(1):e005800. doi: 10.1161/CIRCEP.117.005800

Figure 3. F97C-Cav3 significantly reduces Kir2.2 cell surface expression in HEK293 Cells.

Figure 3

A) The bar graph describes the summary data from multiple FRET experiments between Kir2.2 (GFP tagged) and Cav3 (mCherry tagged), showing that the average FRET efficiency is 25+/−1.76% (n=18) as compared with 6.86+/−1.05% (n=19) in control (Kir2.2 (GFP tagged) + vector (mCherry)) p<0.001. (* indicates p<0.001) B) The bar graph displays quantitative results of eight independent experiments p<0.001. (* indicates p<0.001) C) Representative on-cell Western blot analysis illustrating intensity levels of extracellular HA-tagged Kir2.2 surface expression in wells co-transfected with HA-Kir2.2. Panel (a) shows vector only, Kir2.2 alone panel (b), Kir2.2 with WT-Cav3 (c), and F97C-Cav3 (d). a shows untreated cells stained with both primary and secondary antibody.