- Mix	2.5 μl sense oligo
	2.5 μl antisense oligo
	25.0 μl 2× annealing buffer
	20.0 μl water
- Heat 5 min at 95°C in a dry bath, remove metal block and let block/samples cool down on the bench (to about 40°C). Alternatively, the annealing can be done in a thermo cycler.