| - Mix | 1 μl BsaI-cut vector (20 ng/μl) |
| 1 μl phosphorylated oligos (40 nM) | |
| 1 μl 10× T4 DNA ligase buffer (NEB) | |
| 1 μl T4 DNA ligase (400 units/μl, NEB) | |
| 6 μl water | |
| - Ligate at least 1 hr (or over-night) at room temperature | |
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