Example PCR mix:	5 μl template (10 ng/μl)
	5 μl 10× ThermoPol buffer (NEB)
	0.5 μl dNTPs (25 mM)
	1 μl sense oligo (100 pmol/μl)
	1 μl antisense oligo (100 pmol/μl)
	1 μl Vent DNA Polymerase (2 units/μl, NEB)
	36.5 μl water
Example PCR program:	$\begin{array}{l}\begin{array}{cc}5\text{min}& 94\end{array}°\mathrm{C}\\ \begin{array}{ll}40\text{sec}\hfill & 94°\mathrm{C}\hfill \\ 40\text{sec}\hfill & 52°\mathrm{C}\hfill \\ 60\text{sec}\hfill & 72°\mathrm{C}\hfill \end{array}]35\mathrm{x}\\ \begin{array}{ll}10\text{min}\hfill & 72°\mathrm{C}\hfill \\ \text{∞}\hfill & 4°\mathrm{C}\hfill \end{array}\end{array}$