| - Mix: | 1 μl BamHI/SalI-cut vector (20 ng/μl) |
| 2 μl isolated PCR fragment | |
| 1 μl 10× T4 DNA ligase buffer (NEB) | |
| 1 μl T4 DNA ligase (400 units/μl, NEB) | |
| 5 μl water | |
| - Incubate at least 1 hr (or over-night) at room temperature | |
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