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. Author manuscript; available in PMC: 2018 Jan 17.
Published in final edited form as: Cell Rep. 2017 Dec 26;21(13):3672–3680. doi: 10.1016/j.celrep.2017.12.014

Figure 1. GC Competition Drives the B Cell Response toward Immunodominant Epitopes.

Figure 1

C57BL/6J mice were immunized i.p. with 10 μg of either NP4-OVA or OVA in precipitated alum. Spleens were dissected 12 days after immunization.

(A) Gating strategy for antigen-specific GC B cells (B220+, CD4−, CD38lo, GL7+, OVA+/NP−, or OVA−/NP+) and representative plots from each group.

(B and C) Quantification of antigen-specific B cell frequency (B) and cell count (C).

(D) GC B cell frequency after each immunization.

Cell counts are normalized to 106 lymphocytes. Bars represent mean; NS, not significant; **p < 0.01, ***p < 0.001, unpaired Welch’s t test. See also Figure S1.