Female mice were used for all experiments. (A) ChREBP expression in SQ WAT and liver of 16 week HFD-fed mice (n=5–6/group).
Body weight (B) and fat mass (C) (n=26–27/group) with HFD.
Ad lib-fed DNL from all substrates (D) and glucose (E) in HFD-fed mice (n=6/group).
(F) Terminal wet tissue weight after 16 weeks of HFD (n=5–6/group).
(G) Liver TG content after 8 weeks of HFD (n=5/group). Chow-fed mice (n=2) were used for comparison. #p<0.05 vs. chow,
Ad lib-fed serum insulin (H), FFA (I), TG (J) and adiponectin (AdipoN) (K) levels after 16 week of HFD.
ITT (0.5U/kg, i.p) (L) and GTT (2g/kg, i.p) (M) after 16 weeks of HFD.
n=13–14/group for H–M
(N) Insulin signaling (1U/kg, 15min) after 20 weeks of HFD. (n=3–7/group). #p< 0.05 (Two-Way ANOVA), @p< 0.05 (t-test) vs. saline.
(O) Numbers of total, and pro-inflammatory (M1) and anti-inflammatory (M2) ATMs in PG WAT measured by flow cytometry after 16–20 weeks of HFD (n=5/group).
Data are mean±SEM. *p<0.05 vs. ChREBPfl/fl, same condition; (also see Fig S4)