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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Nat Struct Mol Biol. 2017 Oct 23;24(12):1028–1038. doi: 10.1038/nsmb.3487

Figure 3.

Figure 3

Nucleosome-free linker DNA dictates PRC2 binding to nucleosomes. (a) EMSA of PRC2 binding to H2B-Cy5 labeled nucleosome substrates. Nucleosome core particles lacking an accessible DNA linker (top gel) and mononucleosomes with 60 bp linker DNA (bottom gel), each at 2.5 nM. Binding reactions represent 2-fold titrations of PRC2 ranging from 0–4 µM. (b) PRC2 binds protein-free 147 bp dsDNA > mononucleosome with linker DNA >> nucleosome core particle. Error bars give SD, n = 3 independent experiments. (c) PRC2 protects linker regions of dodecanucleosome arrays from MNase-mediated digestion. M, 123-bp DNA ladder (1 µg per lane) size marker. Gel stained with ethidium bromide. Uncropped gel images are shown in Supplementary Data Set 1.