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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Gastroenterology. 2016 Dec 22;152(5):1002–1013.e9. doi: 10.1053/j.gastro.2016.12.009

Fig. 2. KCC* peptide binding to human colon cells in vitro.

Fig. 2

On confocal fluorescence microscopy, A) intense staining was observed for binding of KCC*-FITC to the surface (arrows) of HT29 cells with a V600E point mutation in BRAF, but not to B) Hs738St/Int (control) cells. C,D) No binding was observed for the PFA*-FITC control peptide to either cell. Labeling the KCC* peptide with either E) biotin or F) Cy5.5 also resulted in strong cell surface binding. Experiments were performed in triplicate.