Figure 1. Overview of sample collection and processing.

See also Table S1 and Table S2. Primary cultures of astrocytes (A–C) or neurons (D–F) loaded with Mitotracker Red with pre- and post- isolation images of target mitochondrion (red arrows) are shown (B vs. C) and (E vs. F). Scale bars in full size images: 20 microns (A,D), in zoomed images: 5 microns (B,C,E,F). Pixels in the zoomed images were resampled for clarity. (G) Schematic of mtDNA coverage by Round 1 and 2 PCR amplicons. Round 2 PCR reactions were pooled (G, Pooled PCR) prior to acoustic shearing (G, Sheared PCR), and standard Illumina library construction (G, DNA libraries).