Table 4. Haplotype frequencies of P. falciparum FC27-like msp2 of from mild, severe and cerebral malaria patients in Thailand, comprising polymorphisms in block 2, 3 and 4.
| FC27 haplotype | Amino acid changesa | Mild (%) |
Severe (%) |
Cerebral (%) |
Total (%) |
M vs Sc P-value, OR |
M vs C P-value, OR |
S vs C P-value, OR |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BI.2 - BI.3 - BI.4 Haplotype 1b |
8 | -9 | -11 | -13 | -16 | -indel | -R1R2 | -20 | -22 |
14 (25.9) |
6 (21.4) |
10 (31.3) |
30 (26.3) |
0.653, 0.78 |
0.595, 1.30 |
0.39, 1.67 |
| K | S | G | N | K | del | A333 | E | Q | ||||||||
| 2 | · | · | · | · | · | del | A33333 | · | · | 0 (0) | 0 (0) | 2 (6.3) | 2 (1.8) | NA. | NA. | NA. |
| 3 | · | · | · | · | · | del | A33333 | · | · | 5 (9.3) | 1 (3.6) | 1 (3.1) | 7 (6.1) | NA. | NA. | NA. |
| 4 | T | · | · | · | · | del | A122 | · | · | 3 (5.6) | 0 (0) | 2 (6.3) | 5 (4.4) | NA. | NA. | NA. |
| 5 | T | · | · | · | N | del | A122 | · | · | 1 (1.9) | 3 (10.7) | 1 (3.1) | 5 (4.4) | NA. | NA. | NA. |
| 6b | T | · | · | · | N | del | A1222 | · | · | 5 (9.3) | 0 (0) | 7 (21.9) | 12 (10.5) | 0.097 | 0.103, 2.75 | 0.009 |
| 7 | T | · | · | · | N | del | A132 | · | · | 2 (3.7) | 1 (3.6) | 0 (0) | 3 (2.6) | NA. | NA. | NA. |
| 8 | T | · | · | · | N | del | A1222 | K | · | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 9 | T | · | · | · | N | del | A12 | K | K | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 10b | T | · | · | · | N | del | A122 | K | K | 8 (14.8) | 4 (14.3) | 2 (6.3) | 14 (12.3) | 0.949, 0.96 | 0.231, 0.38 | 0.301, 0.4 |
| 11 | T | R | · | · | N | del | A122 | · | · | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 12 | N | · | · | · | N | APN | A222 | K | K | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 13 | N | · | · | D | · | del | C2111 | · | · | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 14 | N | · | D | K | · | del | ADD2 | · | · | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 15 | N | · | D | K | · | del | B22 | · | · | 0 (0) | 2 (7.1) | 0 (0) | 2 (1.8) | NA. | NA. | NA. |
| 16 | N | · | D | K | · | del | A222 | · | · | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 17 | N | · | D | K | · | del | B2222 | · | · | 0 (0) | 1 (3.6) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 18 | N | · | D | K | N | del | ADD2 | · | · | 1 (1.9) | 0 (0) | 1 (3.1) | 2 (1.8) | NA. | NA. | NA. |
| 19b | N | R | · | · | · | del | A122 | · | · | 7 (13.0) | 8 (28.6) | 6 (18.8) | 21 (18.4) | 0.083, 2.69 | 0.469, 1.55 | 0.370, 0.58 |
| 20 | N | R | · | · | · | del | A222 | · | · | 1 (1.9) | 0 (0) | 0 (0) | 1 (0.9) | NA. | NA. | NA. |
| 21 | N | R | · | · | N | APK | A1333 | · | · | 0 (0) | 2 (7.1) | 0 (0) | 2 (1.8) | NA | NA. | NA. |
a Position relative to the first aa. of each block (Fig 2).
b Major haplotypes (frequency ≥ 10%) observed in the parasite population that were analyzed for association with malaria severity.
c Haplotype frequencies were compared between mild (M) and severe (S), mild and cerebral (C), and severe and cerebral.
P-value and Odds ratios (OR) are shown, with statistically significant differences in bold. NA. (not applicable) indicates haplotypes with frequencies >10% whose association with malaria severity was not analyzed. OR was undefined in cases of zero cell count.