Table 6. Haplotype frequencies of 3D7 like msp2 of P. falciparum from mild, severe and cerebral malaria patients in Thailand, with each block analyzed separately.
| 3D7 haplotype | Amino acid changesa | Mild (%) |
Severe (%) |
Cerebral (%) |
Total (%) |
M vs Sc P-value, OR |
M vs Cc P-value, OR |
S vs Cc P-value, OR |
|---|---|---|---|---|---|---|---|---|
| Block 2 | 4–5–6 | |||||||
| Haplotype 1b | N P P | 27 (45.0) | 22 (41.5) | 20 (46.5) | 69 (44.2) | 0.709, 0.87 | 0.879, 1.06 | 0.623, 1.23 |
| 2b | · · S | 7 (11.7) | 7 (13.2) | 7 (16.3) | 21 (13.5) | 0.804, 1.15 | 0.501, 1.47 | 0.672, 1.28 |
| 3b | K · · | 13 (21.7) | 17 (32.1) | 9 (20.9) | 39 (25.0) | 0.211, 1.71 | 0.928, 0.96 | 0.222, 0.56 |
| 4b | K T · | 11 (18.3) | 6 (11.3) | 7 (16.3) | 24 (15.4) | 0.298, 0.57 | 0.787, 0.87 | 0.480, 1.52 |
| 5 | K L · | 2 (3.3) | 1 (1.9) | 0 (0) | 3 (1.9) | NA. | NA. | NA. |
| Block 3 NR-R2 | 1_10indel -11–12–14–17–18 - [T] | |||||||
| Haplotype 1b | Ins G E R P P A 8 | 11 (18.0) | 8 (14.3) | 10 (21.7) | 29 (17.8) | 0.583, 0.76 | 0.633, 1.26 | 0.326, 1.67 |
| 2 | Ins G · G S · · 14 | 4 (6.6) | 3 (5.4) | 0 (0) | 7 (4.3) | NA. | NA. | NA. |
| 3 | Ins G · G S · · 11 | 1 (1.6) | 0 (0) | 0 (0) | 1 (0.6) | NA. | NA. | NA. |
| 4 | Ins G · G S R · 11 | 1 (1.6) | 0 (0) | 2 (4.3) | 3 (1.8) | NA. | NA. | NA. |
| 5 | Ins G K · · · · 8 | 1 (1.6) | 1 (1.8) | 1 (2.2) | 3 (1.8) | NA. | NA. | NA. |
| 6 | Ins R · · · · · 11 | 0 (0) | 1 (1.8) | 0 (0) | 1 (0.6) | NA. | NA. | NA. |
| 7 | Ins R · G S · · 8 | 6 (9.8) | 6 (10.7) | 2 (4.3) | 14 (8.6) | NA. | NA. | NA. |
| 8b | Ins R · G S · · 14 | 6 (9.8) | 12 (21.4) | 4 (8.7) | 22 (13.5) | 0.083, 2.50 | 0.841, 0 .87 | 0.079, 0.35 |
| 9 | Ins R · G S · · 11 | 1 (1.6) | 0 (0) | 1 (2.2) | 2 (1.2) | NA. | NA. | NA. |
| 10b | Del3_6 K · · · · 8 | 14 (23.0) | 11 (19.6) | 11 (23.9) | 36 (22.1) | 0.663, 0.82 | 0.907, 1.06 | 0.602, 1.29 |
| 11 | Del1_8 · · · · · 8 | 4 (6.6) | 6 (10.7) | 4 (8.7) | 14 (8.6) | NA. | NA. | NA. |
| 12 | Del1_8 · · · · · 11 | 5 (8.2) | 3 (5.4) | 4 (8.7) | 12 (7.4) | NA. | NA. | NA. |
| 13 | Del1_8 · G S · · 14 | 1 (1.6) | 0 (0) | 0 (0) | 1 (0.6) | NA. | NA. | NA. |
| 14 | Del1_8 K · · · T 8 | 0 (0) | 0 (0) | 1 (2.2) | 1 (0.6) | NA. | NA. | NA. |
| 15b | Del1_10 · G · · · 8 | 6 (9.8) | 5 (8.9) | 6 (13.0) | 17 (10.4) | 0.867, 0.90 | 0.603,1.38 | 0.505, 1.53 |
| Block 4 | 14–17–20–26–28–31–32 | |||||||
| Haplotype 1b | P N E K E K P | 7 (11.5) | 8 (14.3) | 6 (13.0) | 21 (12.9) | 0.650, 1.29 | 0.806, 1.16 | 0.856, 0.90 |
| 2b | · · · · · E Q | 5 (8.2) | 11 (19.6) | 4 (8.7) | 20 (12.3) | 0.072, 2.74 | 0.927, 1.07 | 0.120, 0.39 |
| 3 | · · · · Q E · | 7 (11.5) | 4 (7.1) | 2 (4.3) | 13 (8.0) | NA. | NA. | NA. |
| 4 | · · · N K · · | 1 (1.6) | 0 (0) | 0 (0) | 1 (0.6) | NA. | NA. | NA. |
| 5 | · · · N K E · | 2 (3.3) | 1 (1.8) | 1 (2.2) | 4 (2.5) | NA. | NA. | NA. |
| 6 | · · K · · · · | 6 (9.8) | 5 (8.9) | 5 (10.9) | 16 (9.8) | NA. | NA. | NA. |
| 7 | · · K N G · · | 5 (8.2) | 4 (7.1) | 3 (6.5) | 12 (7.4) | NA. | NA. | NA. |
| 8b | · · K N G E · | 12 (19.7) | 8 (14.3) | 7 (15.2) | 27 (16.6) | 0.439, 0.68 | 0.551, 0.73 | 0.895, 1.08 |
| 9b | · K · · · · · | 5 (8.2) | 7 (12.5) | 11 (23.9) | 23 (14.1) | 0.443, 1.60 | 0.024, 3.52 | 0.132, 2.20 |
| 10 | S · · N · del · | 6 (9.8) | 3 (5.4) | 1 (2.2) | 10 (6.1) | NA. | NA. | NA. |
| 11 | S · · N K · · | 0 (0) | 3 (5.4) | 1 (2.2) | 4 (2.5) | NA. | NA. | NA. |
| 12 | · · · ---- 23_33 del---- | 5 (8.2) | 2 (3.6) | 5 (10.9) | 12 (7.4) | NA. | NA. | NA. |
a Position relative to the first aa. of each block (Fig 3).
b Major haplotypes (frequency ≥ 10%) observed in the parasite population that were analyzed for association with malaria severity.
c Haplotype frequencies were compared between mild (M) and severe (S), mild and cerebral (C), as well as severe and cerebral. P-values and odds ratios (OR) are shown, with significant differences in bold. NA. (not applicable) indicates haplotypes with frequencies >10% whose associations with malaria severity were not analyzed.