Fig 4. Average relative expression (RE) of three transcripts in individual whole female M. domestica injected with 5 μg of dsGFP, dsActin-5C, dsRPL26 and dsRPS6.

Individual expression levels were all normalized to a single dsGFP sample for each replicate. RE for actin-5C, RPL26 and RPS6 were all significantly lower in their respective dsRNA treatment groups when compared to RE in dsGFP treated house flies. Non-target transcripts remained at normal expression levels in all treatment groups indicating a clear RNAi response to exogenous dsRNA. Data represents n = 15 flies total from 3 replicate experiments except dsRPS6 (n = 14).