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Development of in vitro resistance to PAAG was compared to that of two other topical antibiotics, mupirocin and bacitracin, plotted as MIC fold change vs passage number (A). S. aureus ATCC# 6538 developed resistance to PAAG and bacitracin at a slower rate compared to the rapid onset of resistance to mupirocin. Fig 3B is an expansion of Fig 3A showing the MIC fold change to PAAG and Bacitracin.
