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. 2018 Jan 4;14(1):e1006790. doi: 10.1371/journal.ppat.1006790

Fig 2. Validation of miRNAs altered in human DC during Mtb infection.

Fig 2

DC were infected with Mtb for 3, 8 and 24 hours. (A) Time-series plots from array data of miR-155, miR-155*, miR-29b-1*, miR-150, miR-146a, miR-212 and miR-483-5p are shown. The selected miRNAs are all significantly up-regulated (FDR <0.05) during Mtb infection of human DC. When different probes corresponding to the same miRNA were found significant, the mean log2 expression profile across probes was plotted for the two conditions (CTRL and Mtb). (B) Relative expression of miR-155, miR-155*, miR-29b-1*, miR-150, miR-146a, miR-212 and miR-483-5p in human DC left untreated (CTRL) or infected for 3, 8 and 24 hours with wild type Mtb, as measured by q-PCR analysis. Data are derived from 3 healthy donors independently enrolled from those used for microarray and are normalized to the U6b level using the equation 2-ΔCt. The results shown were mean fold change per sample ± standard error of the mean at 3, 8 and 24 hours post-infection (*p = 0.005; **p = 0.02; ***p = 0.04).