Skip to main content
. 2018 Jan 4;14(1):e1006790. doi: 10.1371/journal.ppat.1006790

Fig 5. Identification and biological validation of ATG3 as miR-155 target in DC.

Fig 5

(A) MiR-155 putative binding site in ATG3 3’-UTR region identified by multiple target prediction algorithms. (B) Luciferase assay on HEK293T cells showing specific targeting of 3′-UTR of ATG3 mRNA by the miR-155. Data are represented as the mean fold change per sample ± Standard Deviation of three independent experiments (*p = 0.018). (C) Atg7, Atg3, LC3 and GABARAP protein levels were determined by immunoblotting analysis on un-transfected DC (CTRL) or DC transfected for 24 hours with a miRNA mimic oligonucleotide for miR-155 and with a negative-control miRNA mimic (CTRL mimic). Band intensity quantification for Atg7, Atg3, LC3 and GABARAP is indicated at the bottom of each immunoblot. Actin levels were analyzed to verify the amount of loaded proteins. A representative experiment out of three experiments that yielded similar results is shown.