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. 2018 Jan 4;14(1):e1006821. doi: 10.1371/journal.ppat.1006821

Fig 3. Avian influenza NS1 protein is able to inhibit IFN-β production in A549 cells.

Fig 3

(A) Activation of the IFN-β promoter at 24 hpi in A549-Luc cells after infection with 6:2 RG viruses (MOI = 3). (B, C) IFN-β and IFN-α induction at 24 hpi in MTECs (mouse tracheal epithelial cells) after infection with RG viruses (MOI = 3) determined by ELISA. The dashed line indicates the minimum detection limit. (D-F) Suppression of IFN-β promoter in A549-Luc cells by the Eng/09, Vic /75 or Tky/05 NS1 protein. (D, E) 1000 ng plasmid was transfected followed by infection with influenza or NDV. Data are expressed as mean ± SD (n = 3). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 indicate significant difference compared between 6:2 Eng/09 and Eng/09:TkyNS virus, and between 6:2 Tky/05, 6:2 Eng/09 and 6:2 Vic/75 NS1; unlabeled indicates no significant difference. hpi, hours post infection.