Table 1. Yeast strains used in the study.
| yYB number | Mating type | Genotype |
|---|---|---|
| 7828 | a | nup49::NUP49-GFP:HIS3; his3∆1 leu2∆0 ura3∆0 met15∆0 |
| 4223 | a | nup49::NUP49-GFP:HIS3; bud6::natNT2; his3∆1 leu2∆0 ura3∆0 met15∆0 |
| 10411 | a | sec61::SEC61-GFP:hpnNT1; his3∆0 leu2∆0 met15∆0 ura3∆0 – clone 1 |
| 10412 | a | sec61::SEC61-GFP:hpnNT1; his3∆0 leu2∆0 met15∆0 ura3∆0 – clone 2 |
| 10413 | a | sec61::SEC61-GFP:hpnNT1; his3∆0 leu2∆0 met15∆0 ura3∆0 – clone 3 |
| 1879 | α | sec61::SEC61-GFP:TRP1; shs1::KAN; his3∆200 trp1∆63 leu2∆0 ura3-52 ade2-101 lys2-801 – clone1 |
| 1880 | a | sec61::SEC61-GFP:TRP1; shs1::KAN; his3∆200 trp1∆63 leu2∆0 ura3-52 ade2-101 lys2-801 – clone2 |
| 6770 | a | nup49::NUP49-GFP:HIS3; tor1::TOR1 A1975V: hpnNT1; his3-∆1 leu2∆0 met15∆0 ura3∆0 |
| 4229 | a | nup49::NUP49-GFP:HIS3; slt2::kanMX4; his3∆1 leu2∆0 ura3∆0 met15∆0 |
| 4231 | a |
nup49::NUP49-GFP:HIS3; slt2::kanMX4; his3∆1 leu2∆0 ura3∆0 met15∆0
Plasmid: pYB 1273 (Table 2) carrying PKC1-R398P |
| 10622 | a | nup49::NUP49-GFP:HIS3; bcy1::hphNTI his3∆1 leu2∆0 ura3∆0 met15∆0 – clone 1 |
| 10623 | a | nup49::NUP49-GFP:HIS3; bcy1::hphNTI his3∆1 leu2∆0 ura3∆0 met15∆0 – clone 2 |
| 10624 | a | nup49::NUP49-GFP:HIS3; bcy1::hphNTI his3∆1 leu2∆0 ura3∆0 met15∆0 – clone 3 |
| 4221 | a |
spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; ura3-52 ade2-101 trp1-∆63
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) |
| 6099 | a |
spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; ura3-52
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) |
| 4222 | a |
spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; bud6::natNT2; ura3-52 ade2-101 trp1-∆63
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 1 |
| 5547 | a |
spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; bud6::natNT2; ura3-52 ade2-101 trp1-∆63
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 2 |
| 6521 | a |
spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; bud6::natNT2; ura3-52
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 1 |
| 6522 | a |
spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; bud6::natNT2; ura3-52
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 2 |
| 6523 | a |
spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; bud6::natNT2; ura3-52
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 3 |
| 7301 | α |
nsg1::NSG1-GFP:HIS3; spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; ura3-52
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 1 |
| 7302 | α |
nsg1::NSG1-GFP:HIS3; spc42::SPC42-CFP:kanMX4; trp1::GAL4-EBD:TRP1; leu2::TETR-GFP:LEU2; his3::pGAL-REC:HIS3; ura3-52
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 2 |
| 5532 | a | nsg1::NSG1-GFP:HIS3; his3∆1 leu2∆0 ura3∆0 met15∆0 |
| 6648 | a |
nup82::NUP82-3sfGFP:kanMX4; PURA3-TETR-mCherry:kanMX4; spc42::SPC42-yeGFP:hphNT1; his3::PGAL-REC:HIS3; trp1::GAL4-EBD:TRP1; ade2-101;
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 1 |
| 6649 | a |
nup82::NUP82-3sfGFP:kanMX4; PURA3-TETR-mCherry:kanMX4; spc42::SPC42-yeGFP:hphNT1; his3::PGAL-REC:HIS3; trp1::GAL4-EBD:TRP1; ade2-101;
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 2 |
| 6765 | a |
gcn5::natNT2; nup82::NUP82-3sfGFP:kanMX4; PURA3-TETR-mCherry:kanMX4; spc42::SPC42-yeGFP:hphNT1; his3::PGAL-REC:HIS3; trp1::GAL4-EBD:TRP1; ade2-101;
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 1 |
| 6752 | a |
gcn5::natNT2; nup82::NUP82-3sfGFP:kanMX4; PURA3-TETR-mCherry:kanMX4; spc42::SPC42-yeGFP:hphNT1; his3::PGAL-REC:HIS3; trp1::GAL4-EBD:TRP1; ade2-101;
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2) – clone 2 |
| 3415 | a | ade2::hisG his3 leu2 lys2 ura3Δ0 trp1Δ63 hoΔ::PSCW11-cre-EBD78-NatMX loxP-UBC9-loxP-LEU2 loxP-CDC20-Intron-loxP-HPHMX |
| 3201 | a | bud6::kanMX4; his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| 962 | a | his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| 14902 | a | SPC42-CFP:kanMX4 Gal4-EBD:TRP1 leu2::LEU2 TetR-GFP his::HIS3 pGAL-Cre ura3-52 trp1-∆63 ADE2::NatMX
Plasmid: pPCM14 (224 tetO-REC-URA3-CEN-REC-LEU2); pYB1273 |