Table 1. Experimental data used to fit the model (training set).
Animals heterozygous for null mutations in lin-3 or lin-12 are described in the model by dividing the signal by two (‘half dose’). The EGF level for one overexpression line (JU1107) was set according to the measured lin-3 mRNA level. For other perturbations, the change in signal is included as a fitting parameter, cf. Table S2. * Notch null mutations result in the formation of two anchor cells, thus the effect of suppressing Notch signaling between VPCs it not obvious; however, several lines of evidence suggest that it yields the pattern 33133.
| Experiment | VPC fates (fate or % 1°, 2°, 3°) | References | ||||
|---|---|---|---|---|---|---|
| P4.p | P5.p | P6.p | P7.p | P8.p | ||
| Wild type | 3° | 2° | 1° | 2° | 3° | |
| EGF/Notch mutants | ||||||
|
let-23 mosaic (no EGF receptors in P5/7 .p) |
wild type | (Koga and Ohshima, 1995; Simske et al., 1995) | ||||
| Half dose of EGF (lin-3/+) | wild type | (Ferguson and Horvitz, 1985) | ||||
| Half dose of Notch (lin-12/+) | wild type | (Greenwald et al., 1983) | ||||
| No Notch signaling * | 3° | 3° | 1° | 3° | 3° | (Sundaram and Greenwald, 1993; Shaye and Greenwald, 2002; Komatsu et al., 2008) |
| Notch null mutant, 2 ACs * (described as 2 × WT EGF) |
3° | 1° | 1° | 1° | 3° | (Greenwald et al., 1983) |
|
lin-15 mutant (ectopic EGF; fit to ~0.4 × WT) |
alternating 1° and 2° fates (with or without AC) |
(Sternberg, 1988) | ||||
| EGF overexpression | ||||||
| JU1107 2.75 × WT EGF (based on measured mRNA level) |
2, 16, 82 | 22, 78, 0 | 100, 0, 0 | 16, 84, 0 | 3, 13, 84 | (Barkoulas et al., 2013) |
| JU1100 (mRNA level not determined; level fit) |
24, 54, 21 | 54, 46, 0 | 96, 4, 0 | 37, 63, 0 | 12, 39, 49 | (Hoyos et al., 2011) |
|
AC ablation at successive developmental stages
(mapped to evenly spaced time points in the model) |
(Milloz et al., 2008) | |||||
| L2 lethargus (t = 0.2) | 3° | 3° | 3° | |||
| early L3 (t = 0.32) | 3, 24, 74 | 18, 19, 64 | 0, 20, 80 | |||
| DU divided (t = 0.44) | 0, 59, 41 | 31, 37, 32 | 0, 53, 47 | |||
| VU divided (t = 0.56) | 0, 95, 5 | 53, 48, 0 | 8, 86, 6 | |||
| 3° divided (t = 0.68) | 3, 98, 0 | 65, 35, 0 | 0, 100, 0 | |||
| two-cell stage (t = 0.8) | 1, 99, 0 | 93, 7, 0 | 1, 99, 0 | |||
| Epistasis between EGF/Notch | (Barkoulas et al., 2013) | |||||
| CB1417 (lin-3(e1417) EGF hypomorph; level fit) |
0, 0, 100 | 0, 10, 90 | 54, 0, 46 | 0, 10, 90 | 0, 0, 100 | |
| JU2064 (mild ectopic Notch activity; level fit) |
0, 1, 99 | 0, 100, 0 | 100, 0, 0 | 0, 100, 0 | 1, 2, 97 | |
| JU2095 (mild ectopic Notch activity in the lin-3(e1417) background) |
0, 0, 100 | 1, 14, 86 | 72, 1, 28 | 0, 16, 84 | 0, 2, 98 | |