Figure 4.

Lithium citrate pretreatment prevents neuronal death in the hilus following seizures in vivo. Adult male rats were subjected to pilocarpine-induced seizures with or without a 30-min pretreatment with lithium citrate (6 mg/kg) and injections twice daily for 3 d. A, Sections through the hilus showing double labeling with anti-p75^NTR and anti-CC3. B, Quantification of p75^NTR/CC3 double-labeled cells in the hilus with the different treatments. Cells were counted in every 8^th section through the hippocampus. C, Quantification of p75^NTR/fluorojade-labeled cells in the hilus with the indicated treatments. Scale bars: 10 µm. Quantification in B, C is expressed as percentage control values which are set at 100%. * different from control at p < 0.05, ** different from Pilo at p < 0.05.