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. 2017 Dec 12;122(2):231–245. doi: 10.1161/CIRCRESAHA.117.312392

Figure 5.

Figure 5.

VEC (vascular endothelial cadherin) sequesters Ezh2 (enhancer of zeste homolog 2) at the plasma membrane. A, Coimmunoprecipitation and WB of endogenous Ezh2 and VEC or N-cadherin from extracts of confluent VEC-null and VEC-positive endothelial cells (ECs). B, Coimmunoprecipitation and Western blot (WB) of endogenous Ezh2 and VEC from wild-type (WT) murine whole lung extracts. C, Coimmunoprecipitation and WB of endogenous Ezh2 and VEC from extracts of confluent VEC-null and VEC-positive ECs after biotinylation of cell surface proteins. Asterisk highlights Ezh2-associated total and surface VEC bands. D, Immunofluorescence analysis of Ezh2 junctional localization (arrow) in confluent VEC-null and VEC-positive ECs. Junctional Suz (suppressor of zeste)12 was not detected. Platelet/endothelial cell adhesion molecule-1 (Pecam1) and VEC were used as junctional markers. Scale bar: 10 μm. IP indicates immunoprecipitation; and TL, total cell lysate.