Figure 3. Combination therapy of macrolide and corticosteroid inhibited lung inflammation in murine model of neutrophilic asthma. (A) BALF analysis was performed to evaluate lung inflammation. The number of total cell counts, eosinophil, and neutrophil was significantly increased after OVA+LPS challenge. The number of total cell counts was significantly more decreased in CAM+DXM group compared to decrease in CAM or DXM group. Neutrophils in combination of CAM+DXM treatment group was decreased and it was not seen in CAM or DXM group. The number of eosinophils was not decreased in CAM, DXM, and CAM+DXM group. (B) Accumulation of inflammatory cells improved significantly more in CAM+DXM group compared to DXM group in H&E staining. O+L vs. all (^*p<0.05, ^**p<0.01, ^***p<0.001), O+L+C vs. O+L+D+C (^†p<0.05, ^††p<0.01, ^†††p<0.001), O+L+D vs. O+L+D+C (^‡‡p<0.01, ^‡‡‡p<0.001). BALF: bronchoalveolar lavage fluid; O: ovalbumin (OVA); L: lipopolysaccharide (LPS); C: clarithromycin (CAM); D: dexamethasone (DXM).