Figure 1. Pedigree structure and CLUSTAL alignment of evolutionary conservation in eight families, in whom the steroid-resistant nephrotic syndrome (SRNS)-causing mutation was identified.
(a–h) Pedigrees are shown with proband identified in the pedigree structure. Segregation of genetic variants was examined if affected sibling or parental DNA was available. In (c, d, and f) no CLUSTAL alignment was created as the mutation resulted in a truncating, frameshift mutation, or splice site (intronic), respectively.