Table 2.
Mutation sequencing data from 38 individual CD90-deficient clones derived from the culture of L5178Y/Tk+/− cells treated with EMS
| Litron ID | Primary X- chromosome mutation |
Primary mutation frequency |
Amino acid change |
Litron ID | Primary X- chromosome mutation |
Primary mutation frequency |
Amino acid change |
|---|---|---|---|---|---|---|---|
| a-c3 | 164423363 G>A | 0.99 | Splice site | b2–b4 | 164428596 G>A | 0.99 | Gly>Glu |
| a-c5 | 164423346 G>A | 0.99 | Arg>Lys | b2-c4 | 164423087 C>T | 0.99 | Gln>Stop |
| a-d2† | 164428832 G>A | 0.99 | Gly>Arg | b2-c6 | 164428539 G>A | 0.99 | Splice site |
| a-d2 | 164428832 G>A | 0.99 | Gly>Arg | b2-d1 | 164428833 G>A | 0.99 | Gly>Glu |
| a-d3 | 164428004 G>A | 0.99 | Gly>Glu | b2-d2 | 164428833 G>A | 0.99 | Gly>Glu |
| a-d5 | 164428010 G>A | 0.99 | Gly>Glu | b3-a5 | 164428833 G>A | 0.99 | Gly>Glu |
| a1–a5 | 164423003 G>A | 0.99 | Glu>Lys | c1-a1 | 164428617 C>T | 0.99 | Ser>Phe |
| a1-b3 | 164423003 G>A | 0.99 | Glu>Lys | c1-a2 | 164423021 C>T | 0.99 | His>Tyr |
| a1-d1 | 164423109 C>T | 0.99 | Ser>Phe | c1-a3 | 164428932 C>A | 0.99 | Ser>Stop |
| b1-a3 | 164423363 G>A | 0.84* | Splice site | c1-a6 | 164427979 C>T | 0.99 | Gln>Stop |
| b1-a5 | 164423363 G>A | 0.78** | Splice site | c1-b1 | 164428004 G>A | 0.99 | Gly>Glu |
| b1-a6 | 164423363 G>A | 0.99 | Splice site | c1-c1 | 164423363 G>A | 0.99 | Splice site |
| b1–b5 | 164428596 G>A | 0.99 | Gly>Glu | c1–c2 | 164423021 C>T | 0.99 | His>Tyr |
| b1-c1 | 164423363 G>A | 0.99 | Splice site | c1–c6 | 164428617 C>T | 0.99 | Ser>Phe |
| b1-c6 | 164428009 G>A | 0.99 | Gly>Arg | c1-d1 | 164423021 C>T | 0.99 | His>Tyr |
| b1-d2 | 164428596 G>A | 0.99 | Gly>Glu | c1-d4 | 164428832 G>A | 0.99 | Gly>Arg |
| b1-d3 | 164428596 G>A | 0.99 | Gly>Glu | c3-a4 | 164428004 G>A | 0.99 | Gly>Glu |
| b2-a1 | 164428670 C>T | 0.99 | Gln>Stop | c3–c4 | 164427932 G>A | 0.99 | Splice site |
| b2-a4 | 164428009 G>A | 0.99 | Gly>Arg | c3-d2 | 164427979 C>T | 0.99 | Gln>Stop |
| b2-b1 | 164428989 G>A | 0.99 | Trp>Stop | L5178Y | None | --- | None |
*
A secondary mutation at 164428670 C>T (Gln>Stop, 0.16 frequency) was present in this clone.
This secondary mutation was found as a primary mutation in other clones.
**
A secondary mutation at 164428810 G>A (Splice site, 0.21 frequency) was present in this clone.
This secondary mutation was not found as a primary mutation in other clones.
†
Duplicate samples of this clone were submitted, both showed same results.