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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Cancer Res. 2017 Nov 29;78(2):422–435. doi: 10.1158/0008-5472.CAN-17-1533

Fig 5. MED1 LxxLL motif mutations impaired MMTV-HER2 cancer stem cell formation and function both in vitro and in vivo.

Fig 5

(A–B) Flow cytometry analyses of Lin−CD24+CD29hi CSCs enriched population in MMTV-HER2/MED1+/+ and MMTV-HER2/MED1KI/KI primary tumors (A) and quantification (B). (C) Mammosphere formation assays using the same number of above FACS sorted cells (D–E) Quantification of the size (D) and member (E) of mammospheres formed in (C). (F) Representative tumor bearing NOD-SCID mice at 3month post orthotopic transplantation of indicated number (labeled above) of MMTV-HER2/MED1KI/KI (blue circle) and MMTV-HER2/MED1+/+ (red circle) tumor cells. (G) Statistical analyses of tumor formation in each group of (F). (H–I) Flow cytometry analysis of Lin−CD24+CD29hi CSCs enriched population in orthotopically xenografted tumors (H) and quantification (I). Bar = 100 µm. The values are obtained from three independent experiments and shown as mean ± SD. *P < 0.05 or **P < 0.01.