Skip to main content
. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Biochemistry. 2017 Nov 8;57(2):226–230. doi: 10.1021/acs.biochem.7b01021

Figure 1.

Figure 1

Strategy for using propargylcholine (ProCho) as a reporter for PLD activity and for PC biosynthesis. (A) Abbreviated biosynthetic pathways for phosphatidylcholine (PC) synthesis and phospholipase D (PLD)-mediated phosphatidic acid (PA) production. (B) Established strategy for using ProCho as a reporter for PC biosynthesis.11 (C) Established strategy for using small, linear, clickable primary alcohols such as 3-azido-1-propanol (AzProp) as reporters for PLD activity.16,17 (D) Strategy for repurposing ProCho as a reporter of PLD activity by controlling labeling conditions as indicated. Other abbreviations: Cho, choline; Phos-Cho, phosphocholine; CDP-Cho, cytidine 5′-diphosphocholine; DAG, diacylglycerol; CuAAC, copper-catalyzed azide–alkyne cycloaddition; SPAAC, strain-promoted azide–alkyne cycloaddition.