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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Dev Dyn. 2017 Dec 4;247(2):304–314. doi: 10.1002/dvdy.24605

Fig 3. Pten deficiency causes enhanced osteoblast differentiation and neurogenesis.

Fig 3

(A–H) AP-AB staining on frontal sections of littermate control and Ptenfl/fl; Wnt1Cre2 embryos at E13.5 (A–D) and at E16.5 (E–H). Sections were counterstained with nuclear fast red. (I) Quantification result of osteogenesis markers expression of the control and Ptenfl/fl; Wnt1Cre2 mRNA samples prepared from E13.5 embryos (n=3). (J, K) In situ hybridization with Osteocalcin antisense probe on the frontal sections of the control (J) and Ptenfl/fl; Wnt1Cre2 (K) embryos at E15.5. (L–O) Immunostaining with anti-Neurofilament-L antibody on frontal sections of littermate control (L, N) and Ptenfl/fl; Wnt1Cre2 (M, O) embryos at E13.5. fb, frontal bone; md, mandible; mn, maxillary nerve; pb, palatine bone; pmx, premaxilla; and tgg, trigeminal ganglion.