Skip to main content
. Author manuscript; available in PMC: 2018 Jun 11.
Published in final edited form as: Nat Chem Biol. 2017 Dec 11;14(2):135–141. doi: 10.1038/nchembio.2534

Figure 2. Genetic evidence for Mir1 as primary target of ML316.

Figure 2

(a) ML316-sensitivity is correlated with MIR1 gene dosage and mutation status. Antifungal susceptibility testing with ML316 was performed using wild type and genetically modified C. albicans strains. Relative growth as monitored by OD600 was normalized to untreated control for each strain and is displayed in heat map format. Data represent the mean of results from two independent experiments. (b and c) ML316 treatment phenocopies the deletion of MIR1 in S. cerevisiae. Change in optical density over time as a measure of growth was monitored in liquid cultures of wild type S. cerevisiae and the indicated mutant strains. Assays were performed in (b) glucose- or (c) galactose-supplemented synthetic media with vehicle (DMSO) or ML316 (500nM) added as indicated. Results are from one of two independent experiments. The mean of measurements from 4 independent wells is depicted.