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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Cancer Res. 2017 Nov 27;78(2):572–583. doi: 10.1158/0008-5472.CAN-17-2040

Figure 2.

Figure 2

Ascorbate treatment reduces H4ac but not H3ac in melanoma cells. A, Immunoblot of H3ac and H4ac in A2058 and 1205Lu cells treated with or without ascorbate (50 μM) for 72 hours. Total H3 and H4 were used for loading controls. B, Densitometry analysis of H3ac normalized by total H3. No significant change in H3ac was observed after ascorbate treatment. C, Densitometry analysis of H4ac normalized by total H4. The levels of H4ac were reduced by ascorbate treatment in A2058 and1205Lu cells. D–F, Immunofluorescence and quantification of H3ac in A2058 and 1205Lu cells treated with or without ascorbate (50 μM) for 72 hours. No significant change in H3ac was observed in A2058 and 1205Lu cells. G–I, Immunofluorescence and quantification of H4ac in A2058 and 1205Lu cells treated with or without ascorbate (50 μM) for 72 hours. H4ac was decreased by ascorbate treatment in A2058 and 1205Lu cells. All data are mean ± SEM.

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