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. 2017 Dec 5;8:27–40. doi: 10.1016/j.omto.2017.12.002

Figure 2.

Figure 2

Characterization of In Vitro Infection and Replication of the Mutant Deletion Viruses

(A) Plaque size was measured in a BSC-40 monolayer (solid agar medium) at 48 hpi (MOI 0.005 PFU/cell) and the area given in a.u. (B–E) Replication capacity was analyzed through viral growth curves by recovering infected monolayers (MOI 0.01 PFU/cell) at different time points (0, 8, 24, 32, 48, and 72 hpi) followed by titration; the cell lines used were (B) CEF, (C) BSC-40, (D) B16F10, and (E) TRAMP-C1. (F) Spheroids of TRAMP-C1 cells were infected (MOI 2 PFU/cell) and observed after 6 days. Left: white light; center: fluorescence filter for GFP; right: H&E-stained histological sections. Scale bars, 500 μm. Data are shown as mean ± SD. **p < 0.01; ***p < 0.005.