Fig. 3.

Cytokine and chemokine profiling. A 29-plex-cytokine magnetic bead assay was performed on a plasma isolated from ZIKV-infected and control fetal and cord blood, b amniotic fluid; and c fetal and maternal cerebral spinal fluid at 135dGA. Plasma analysis revealed changes in the cytokines IL-RA, IL-1β, IL-2, IL-12, IFNγ, and MIF; and chemokines MCP-1 (CCL-2), MDC (CCL-22), IP-10 (CXCL-10), and ITAC (CXCL-11). Compared to controls increased levels of IL-6, Eotaxin (CCL-11), MCP-1 (CCL-2), MIF and FGF-basic were detected in amniotic fluid from infected animals. Levels of IL-1RA, Eotaxin (CCL-11), MCP-1 (CCL-2), MIF, and IP-10 (CXCL-10) were higher in fetal vs. maternal CSF. One-way ANOVA was performed with Tukey’s multiple comparison test, bars represent mean and SEM, **p < 0.01, *p < 0.05 UV, umbilical vein; UC, umbilical cord