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. 2018 Jan 17;8:949. doi: 10.1038/s41598-018-19264-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondrial membrane potential of the Mt iPS cell clones, as measured by FACS analysis. Representative histograms of TMRE intensity among the Mt1 iPS cell clones ((a): 1-1^low, (b): 1-2^low, (c): 1-3^high, (d): 1-4^high) and the Mt2 iPS cell clones ((e): 2-3^low, (f): 2-8^low, (g): 2-6^high, (h): 2-5^high) are shown. The Mt iPS cells were treated with 4 μM FCCP or DMSO for 10 min and analyzed independently. The TMRE peak decreased after FCCP administration was statistically significantly lower in Mt1-4^high than in Mt1-1^low among the Mt1 iPS cell clones, while it was not statistically significantly different among the Mt2 iPS cell clones assessed using ANOVA with a post hoc test (Tukey’s test).