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. 2018 Jan 17;8:949. doi: 10.1038/s41598-018-19264-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-lapse imaging of the Mt iPS cell clones expressing ATeam1.03, a FRET-based ATP biosensor. The time course of the YFP/CFP emission ratio (a) and the sequential images (b) in a single iPS cell of Mt1-1^low expressing ATeam 1.03 were examined after injection of 2DG. 2DG was injected at 10 min. The representative data are expressed as the means ± SD. n = 6 (Scale bar, 10 μm). The YFP/CFP emission ratio of the Mt1 iPS cell clones (c) and the Mt2 iPS cell clones (d) at 10 min (before the oligomycin A injection) and 30 min (20 min after the oligomycin A injection) was measured. The YFP/CFP emission ratio of the Mt1 iPS cell clones (e) and the Mt2 iPS cell clones (f) at 10 min (before 2DG injection) and 40 min (30 min after 2DG injection) was measured. The data are expressed as the means ± SE. n = 10–14 (c) (d). n = 11–16 (e) (f). **P < 0.01 and *P < 0.05, among the 2DG groups assessed by ANOVA with a post hoc test (Tukey’s test). ^#P < 0.01, compared with basal for each clone assessed using the Student’s t-test.